Journal: Viruses

Article Title: Characterization of a Broadly Neutralizing Monoclonal Antibody against SARS-CoV-2 Variants

doi: 10.3390/v14020230

Figure Lengend Snippet: Analysis of mAb 9G8 blocking the SARS-CoV-2 spike RBD variant from binding to the human ACE2 receptor by surrogate virus neutralization test (sVNT). The percent inhibition of mAb 9G8 was measured against RBD of SARS-CoV-2 WT or SARS-CoV-2 variants such as Alpha (B.1.1.7), Beta (B.1.351), Gamma (P.1), Kappa (B.1.617.1), Delta (B.1.617.2), Delta Plus/AY.1 (B.1.617.2.1), and Epsilon (B.1.429/7). The mAb 6C8 specific for nucleocapsid protein was used as a negative control. The results were expressed as the arithmetic mean of percent-blocking values of triplicates and ±SD of the mean.

Article Snippet: To determine the neutralizing activity of mAbs against SARS-CoV-2 variants, a GenScript cPASS surrogate virus neutralization test (sVNT) was performed according to manufacturer’s instructions (GenScript, Piscataway, NJ, USA).

Techniques: Blocking Assay, Variant Assay, Binding Assay, Virus, Neutralization, Inhibition, Negative Control

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Estimating the Neutralizing Effect and Titer Correlation of Semi-Quantitative Anti-SARS-CoV-2 Antibody Immunoassays

doi: 10.3389/fcimb.2022.822599

Figure Lengend Snippet: Analytical performance of each kit in discriminating SARS-CoV-2 infection.

Article Snippet: The ROC AUCs for the prediction of PRNT ND 50 ≥ 20 of the GenScript cPass sVNT, Roche Elecsys Anti-SARS-CoV-2, Roche Elecsys Anti-SARS-CoV-2 S, and Abbott SARS-CoV-2 IgG II Quant kits were 0.941, 0.916, 0.950, and 0.964, respectively ( ).

Techniques: Infection

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Estimating the Neutralizing Effect and Titer Correlation of Semi-Quantitative Anti-SARS-CoV-2 Antibody Immunoassays

doi: 10.3389/fcimb.2022.822599

Figure Lengend Snippet: Analytical performance for representativeness of neutralizing activity using the pre-defined cut-off values of each immunoassay kit.

Article Snippet: The ROC AUCs for the prediction of PRNT ND 50 ≥ 20 of the GenScript cPass sVNT, Roche Elecsys Anti-SARS-CoV-2, Roche Elecsys Anti-SARS-CoV-2 S, and Abbott SARS-CoV-2 IgG II Quant kits were 0.941, 0.916, 0.950, and 0.964, respectively ( ).

Techniques: Activity Assay

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Estimating the Neutralizing Effect and Titer Correlation of Semi-Quantitative Anti-SARS-CoV-2 Antibody Immunoassays

doi: 10.3389/fcimb.2022.822599

Figure Lengend Snippet: Titer correlation of the analytical performance of the prediction of neutralizing activity using newly calculated cut-off values determined using Youden’s index.

Article Snippet: The ROC AUCs for the prediction of PRNT ND 50 ≥ 20 of the GenScript cPass sVNT, Roche Elecsys Anti-SARS-CoV-2, Roche Elecsys Anti-SARS-CoV-2 S, and Abbott SARS-CoV-2 IgG II Quant kits were 0.941, 0.916, 0.950, and 0.964, respectively ( ).

Techniques: Activity Assay

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Estimating the Neutralizing Effect and Titer Correlation of Semi-Quantitative Anti-SARS-CoV-2 Antibody Immunoassays

doi: 10.3389/fcimb.2022.822599

Figure Lengend Snippet: Serial kinetics of antibody titers measured with each method: (A) PRNT ND50, (B.) GenScript cPass sVNT, (C) Roche Elecsys Anti-SARS-CoV-2, (D) Roche Elecsys Anti-SARS-CoV2 S, and (E) Abbott AdviseDx SARS-CoV2 IgG II.

Article Snippet: The ROC AUCs for the prediction of PRNT ND 50 ≥ 20 of the GenScript cPass sVNT, Roche Elecsys Anti-SARS-CoV-2, Roche Elecsys Anti-SARS-CoV-2 S, and Abbott SARS-CoV-2 IgG II Quant kits were 0.941, 0.916, 0.950, and 0.964, respectively ( ).

Techniques:

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Estimating the Neutralizing Effect and Titer Correlation of Semi-Quantitative Anti-SARS-CoV-2 Antibody Immunoassays

doi: 10.3389/fcimb.2022.822599

Figure Lengend Snippet: Antibody titers by timeline.

Article Snippet: The ROC AUCs for the prediction of PRNT ND 50 ≥ 20 of the GenScript cPass sVNT, Roche Elecsys Anti-SARS-CoV-2, Roche Elecsys Anti-SARS-CoV-2 S, and Abbott SARS-CoV-2 IgG II Quant kits were 0.941, 0.916, 0.950, and 0.964, respectively ( ).

Techniques:

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Estimating the Neutralizing Effect and Titer Correlation of Semi-Quantitative Anti-SARS-CoV-2 Antibody Immunoassays

doi: 10.3389/fcimb.2022.822599

Figure Lengend Snippet: Scatter plot and Pearson’s correlation for each method grouped with acute/convalescent phase. (A) GenScript cPass sVNT, (B) Roche Elecsys Anti-SARS-CoV-2, (C) Roche Elecsys Anti-SARS-CoV2 S, and (D) Abbott AdviseDx SARS-CoV2 IgG II were compared with PRNT, respectively. Each colored line depicts the linear regression model and the surrounding grey-colored area represents the 95% confidence interval.

Article Snippet: The ROC AUCs for the prediction of PRNT ND 50 ≥ 20 of the GenScript cPass sVNT, Roche Elecsys Anti-SARS-CoV-2, Roche Elecsys Anti-SARS-CoV-2 S, and Abbott SARS-CoV-2 IgG II Quant kits were 0.941, 0.916, 0.950, and 0.964, respectively ( ).

Techniques: